National Institute of Pharmaceutical Education and Research (NIPER), S.A.S. Nagar as an establishment is registered with Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Ministry of Environment, Forest & Climatic Change, Government of India for the Research for education, Research for the commercial purpose, Breeding for in-house use and Breeding for the purpose of trading of small laboratory animals (108/GO/Re/Rc/Bi/Bt/99/CPCSEA). The Central Animal Facility (CAF) is the double storied building with ‘Two-way corridor system’ to minimize the cross contamination and for the efficient animal house operations. The first floor is dedicated to the breeding of different small laboratory rodents like mice, rats, hamsters, gerbils and guinea pigs for the supply to the various in-house and consultancy research projects. The animals are also supplied on request to the outside CPCSEA registered establishments on stipulated terms and conditions. Each animal species is housed separately in individual room to prevent inter-species disease transmission and anxiety due to interspecies conflict. The animals are well maintained under controlled environmental conditions (temperature (23+3°C), relative humidity (30-70 %), 12:12 h light and dark cycle with 100 % of fresh air exchange in animal rooms. The macro- and micro-environment around the animals are maintained as per the CPCSEA guidelines. In addition to the breeding unit, there is a separate experimental unit available for the holding and conducting the experiments on animals. Full time veterinarian is available to provide veterinary care to the animals. A high degree of personal hygiene and sanitation is being practiced. Regular cleaning and disinfection of animal rooms, corridors, storage spaces and other areas are carried out. Routine cleaning and sterilization of animal supplies like cages, water bottles and bedding are performed. Horizontal heavy duty autoclave is installed for the sterilization purpose and regularly monitored for its effectiveness of operations. Periodic health monitoring of the animals is carried out. In addition, feed and water analysis are carried out for ensuring their quality and microbiological contamination. Waste disposal is carried out through incinerator. The routine works at CAF are carried out as per the standard operating procedures adopting GLP principles to achieve the quality production and supply of the experimental animals.